Pharmacology Mechanism of Polygonum Bistorta in Treating Ulcerative Colitis Based on Network Pharmacology

Aim. Ulcerative colitis (UC) is a refractory gastrointestinal disease. The study aimed to expound the mechanism of Polygonum bistorta (PB) in treating UC by network pharmacology, molecular docking, and experiment verification. Methods. compositions targets PB UC-associated were obtained searching websites literature. potential treatment was predicted protein-protein interaction construction, Gene Ontology (GO), Kyoto Encyclopedia Genes Genomes (KEGG) pathway enrichment analysis. Molecule docking performed AutoDock. In vitro experiments explored quercetin (Que), main active composition PB, UC. Results. Six compositions, 139 targets, 934 obtained. 93 overlapping between identified, 18 them core targets. 467 biological processes, 10 cell components, 30 functions GO 102 pathways enriched through KEGG Among them, IL-17 signaling had high importance. FOS, JUN, IL-1β, CCL2, CXCL8, MMP9 could dock with Que successfully. Act1, TRAF6, JUN identified as key proteins pathway. expressions abovementioned increased Caco-2 cells stimulated Dextran sulfate sodium decreased after being treated Que. Conclusion. might treat downregulating It worth doing further research on vivo.